[Pollution Properties and Ecological Risk Assessment of Heavy Metals in Farmland Soils and Crops Around a Typical Manganese Mining Area].

In order to assess the ecological risks of heavy metals and explore the pattern of heavy metal migration between farmland and corresponding crops in a typical and closed manganese mining area in Hunan province, farmland soils and crops surrounding the mining area (pollution area) and away from the mining area (control area) were collected, and then the contents of Cr, Mn, Ni, Cu, Zn, As, Cd, and Pb were analyzed. The sources and distribution of heavy metals in farmland soils were analyzed using Kriging spatial interpolation and principal component analysis, and the ecological risk was evaluated using the single factor index, comprehensive pollution index, and potential ecological risk index. The results showed that the surrounding farmland soils in the closed Manganese mining area presented serious pollution of Cd, Zn, As, and Mn, in which the average contents of the above heavy metals in the dry land soil in the polluted area were 6.22, 612.28, 37.72, and 1506.2 mg·kg-1, respectively. Compared with the soil risk screening value of agricultural land, the over-standard rates of Cd, Zn, and As were 88.41%, 94.20%, and 84.06%, respectively, and the average content of Mn in the farmland soil was three times that of the background value in the Hunan soil; however, the heavy metal pollution in the paddy field was relatively light. The principal component analysis showed that the sources of Cd, Mn, and Zn in the farmland soil were related to the manganese ore mining, whereas the source of As in the farmland soil might originate from agricultural activities. The pollution area was at a heavy pollution level, and the main pollution factors were Cd, Mn, and Zn. The Cd in the farmland soil could pose a strong potential ecological risk, but the rest of the heavy metals presented only a slight potential ecological risk. The content of Cr, Pb, and Cd in the crops in the study area exceeded the standard, and the exceeding standard rate was between 1.1% and 37.3%, where the average content of over-standard heavy metals in corn was higher than that in rice, and the average content of heavy metals in leafy vegetables was higher than that in root vegetables. The soil pollution degree of heavy metals could affect the accumulation ability of crops, and different crops had different accumulation abilities. For instance, leafy vegetables and root vegetables easily accumulated Cd and Zn; however, rice and corn separately enriched Cd and Cr, as well as Zn and Cu. The contents of heavy metals in dryland soils had a positive correlation with the content of heavy metals in corresponding crops. The contents of Cd and As in the paddy field and rice presented a positive correlation, but the remaining six heavy metal contents in rice (i.e., Cr, Mn, Ni, Cu, Zn, and Pb) did not correlate with the content of the paddy fields.

Exosomal miR-214-5p Released from Glioblastoma Cells Modulates Inflammatory Response of Microglia after Lipopolysaccharide Stimulation through Targeting CXCR5.

BACKGROUND AND OBJECTIVE: Exosomes communicate inter-cellularly and miRNAs play critical roles in this scenario. MiR-214-5p was implicated in multiple tumors with diverse functions uncovered. However, whether miR-214-5p is mechanistically involved in glioblastoma, especially via exosomal pathway, is still elusive. Here we sought to comprehensively address the critical role of exosomal miR-214-5p in glioblastoma (GBM) microenvironment. METHODS: The relative expression of miR-214-5p was determined by real-time PCR. Cell viability and migration were measured by MTT and transwell chamber assays, respectively. The secretory cytokines were measured with ELISA kits. The regulatory effect of miR-214-5p on CXCR5 expression was interrogated by luciferase reporter assay. Protein level was analyzed by Western blot. RESULTS: We demonstrated that miR-214-5p was aberrantly overexpressed in GBM and associated with poorer clinical prognosis. High level of miR-214-5p significantly contributed to cell proliferation and migration. GBM-derived exosomal miR-214-5p promoted inflammatory response in primary microglia upon lipopolysaccharide challenge. We further identified CXCR5 as the direct target of miR-214- 5p in this setting. CONCLUSION: Overexpression of miR-214-5p in GBM modulated the inflammatory response in microglia via exosomal transfer.

Solvo-thermal synthesis of a unique alkaline earth-transition Ba-Cd micro-porous coordination framework as hetero-metallic luminescent sensor for Cu2+ and real-time detection of benzaldehyde.

In this work a unique hetero-metallic alkaline earth-transition Ba-Cd luminescent micro-porous metal-organic framework {[BaCd(µ6-tp)1.5(µ2-Cl)(H2O) (DMF)2]·0.75H2O}n (H2tp=terephthalic acid) (1) has been prepared under solvo-thermal conditions. In 1 infinite 1D {Ba-X-Cd} (X=O, Cl) inorganic chains are linked via these full de-pronated tp2- ligands forming a unique 3D I1O2 type micro-porous coordination framework. PXRD patterns of 1 have been determined confirming pure phases of 1. Luminescence investigations suggested that 1 exhibits highly selective and sensitive sensing for trace amounts of benzaldehyde in ethanol, which provides a facile method for real-time detection of benzaldehyde. Meanwhile 1 also exhibits highly selective and sensitive sensing for Cu2+ over other cations with high quenching efficiency Ksv value 1.15×104L·mol-1. As far as we know, 1 represents the first example of alkaline earth-transition hetero-metallic Ba-Cd micro-porous coordination framework as bi-functional luminescent probes for Cu2+ and benzaldehyde.